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Feasibility of a randomized single-blind crossover trial to assess the effects of the second-generation slow-release dopamine agonists pramipexole and ropinirole on cued recall memory in idiopathic mild or moderate Parkinson’s disease without cognitive impairment

  • The Erratum to this article has been published in Pilot and Feasibility Studies 2017 3:48



The aim was to assess the feasibility of a single-centre, single-blind, randomized, crossover design to explore the effects of two slow-release dopamine agonists, ropinirole and pramipexole, on cued recall in Parkinson’s disease.

As the design required a switch from the prescribed agonist (pramipexole-to-ropinirole, or ropinirole-to-pramipexole), the primary objectives were to (a) examine the efficacy of processes and procedures used to manage symptoms during the washout period and (b) to use cued recall estimates to inform a power calculation for a definitive trial.

Secondary objectives were to assess consent and missing data rates, acceptability of clinical support for the OFF sessions, experience of the OFF sessions and of agonist switching, barriers-to-participation for patients and informal caregivers.


Patients were randomized in a 1:1 ratio to two treatment arms and stabilized on each agonist for 6 weeks. The arms differed only in the sequence in which the agonists were administered. Cued recall was assessed ON medication and, following a washout period resulting in 93.75% agonist elimination, OFF medication.


A total of 220 patients were screened: 145 were excluded and 75 invitations to participate were sent to eligible patients. Fifty-three patients declined, 22 consented and 16 completed the study.

There were no serious adverse events, and rates of non-serious adverse events were equivalent between the agonists.

Using the largest standard deviation (SD) of the ON–OFF difference cued recall score (inflated by ~25% to give a conservative estimate of the SD in a definitive trial) and assuming an effect of at least 10% of the observed range of OFF medication cued recall scores for either agonist to be clinically important, a main trial requires a sample size of just under 150 patients.

The consent and missing data rates were 29 and 27% respectively. The washout period and the preparation for the OFF sessions were acceptable, and the sessions were manageable. The experience of switching was also manageable. Barriers to participation included concerns about disease stability, side effects, research process, carer workload and accessibility of the information sheet.


This study presented challenges to recruitment both in design and execution, and while it was a major aim of the study to assess this, evaluation of these challenges provided the opportunity to explore how they could be overcome for future studies.

Trial registration

EudraCT 2012-000801-64

Peer Review reports


Around 10,000 new cases of Parkinson’s disease (PD) are diagnosed each year, which indicates that around 1% of the UK population (an estimated 122,795 people) over the age of 60 years have PD; this figure is set to rise by an estimated 28% by 2020 as the population ages [1,2,3,4]. PD is diagnosed according to the presence of motor features (tremor, rigidity, slowed movement) caused by the loss of dopamine-producing cells in the midbrain [5,6,7,8,9].

Clinical management is focused on using dopaminergic replacement drugs to alleviate the cardinal motor signs. These drugs remediate motor features by increasing availability of dopamine using levodopa and/or monoamine oxidase inhibitors, and/or by mimicking the action of dopamine using second-generation dopamine agonists such as immediate- or slow-release preparations of pramipexole or ropinirole [10,11,12,13,14,15].

Dopaminergic medication can also have a significant effect on cognitive function. Evidence indicates that the requisite dopaminergic state necessary to control motor signs has the potential to move a patient away from his or her optimum for certain cognitive functions [16,17,18,19,20]. The relationship between the efficiency of neuronal activity and the state of dopaminergic modulation in the l-dopa overdose hypothesis is represented by a Yerkes–Dodson inverted U-shaped curve, with cognitive functions declining with deviation away from optimum dopamine levels, indicated by the centre of the curve. Extrapolating this model to cued recall implies that dopaminergic medication has the capacity to both improve and impair this kind of memory, depending on baseline dopamine levels in the underlying neural circuitry.

We have recently reported a series of studies in which cued recall of recently experienced events is impaired in medicated patients with PD [21,22,23,24,25]. Interpreting these findings in the context of the l-dopa overdose hypothesis theory implies at least two competing explanations: either dopaminergic medication is overdosing ‘relatively normal’ dopamine levels in those brain areas supporting memory or dopaminergic drugs are reducing the severity of a PD-related dopamine depletion in the brain areas supporting memory.

We explored these alternative predictions in an open-label, nonrandomized pilot of 24 patients with PD in the early (n = 12) and moderate (n = 12) disease stages [26]. The patients’ memory was compared to that of a group of 24 matched healthy controls. To compare the effects of medication on memory, testing took place when the patients were in a medicated or ‘ON’ state, and also on a different occasion following a 24-hour washout period before the patients took their next medication, and so were in an unmedicated or ‘OFF’ state. Memory was also assessed on 2 occasions in the healthy controls, who were not taking any medication, to control for practice effects. The pilot revealed three key findings:

Firstly, compared to the healthy control performance, the PD group exhibited a cued recall deficit when tested OFF medication, indicating a PD-related memory decline.

Secondly, the severity of the patients’ memory decline increased when the same patients were tested ON medication, suggesting that dopaminergic medication increases the severity of a PD-related decline in memory.

Thirdly, the ON medication decline in memory was most pronounced in a subset of patients medicated with the second-generation non-ergot dopamine agonist pramipexole prolonged-release (abbreviated subsequently to pramipexole), with patients on the second-generation non-ergot dopamine agonist ropinirole modified-release (abbreviated to ropinirole) showing a less severe decline. These preliminary findings were replicated in a further open-label nonrandomized, parallel-group study of 21 patients with PD medicated with either pramipexole or ropinirole [27].

While these were intriguing findings that had not previously been reported in either the scientific or medical literature, the study design meant that the effects of confounding variables, such as demographic and or clinical differences between the patients on either pramipexole or ropinirole, could not be ruled out.

Thus, the aim of this study was to assess the feasibility of a crossover design to explore the effects of pramipexole and ropinirole on memory in patients with idiopathic PD without cognitive impairment.

As the design required patients to switch from their prescribed dopamine agonist (pramipexole-to-ropinirole, or ropinirole-to-pramipexole), the primary objectives were to (a) examine the efficacy of processes and procedures used to manage symptoms during the washout period and (b) to use cued recall estimates to inform a power calculation for a definitive trial.

The secondary objectives were to assess (i) rates of consent and missing data; (ii) acceptability of the support provided for the OFF medication sessions and whether the OFF session itself was manageable; (iii) the experience of switching between the investigational medicinal products (IMPs), i.e. ropinirole and pramipexole, assessed at the mid- and end-of-study clinic visits and in the semi-structured interview administered at the end of the trial; and (iv) barriers to participation for eligible patients and informal carer givers.


The reporting of the study follows the CONSORT statement recommendations [28].


This 24-month feasibility study was a single-centre, single-blind, randomized study of two IMPs, ropinirole and pramipexole, in male and female patients with a confirmed primary diagnosis of idiopathic PD without cognitive impairment.

Patients were recruited on one of these agonists, and randomized in a 1:1 ratio to two treatment arms. The arms differed only in the sequence the IMPs were administered (pramipexole then ropinirole, or ropinirole then pramipexole).

Patient-participants commenced their first trial IMP without a ‘run-in’ or washout period. They were stabilized on this IMP for 6 weeks, and then, their memory was tested on two separate occasions in the following 2 weeks: in one session, the memory was tested 90 min after taking the IMP (ON), and on a separate occasion, the memory was assessed following 24–48 h of IMP withdrawal (OFF).

The OFF session was preceded by a withdrawal period that resulted in 93.75% (four half-lives) IMP elimination. After four half-lives, the amount of drug remaining (6.25%) is considered to be negligible regarding its therapeutic effects [29].

The order of ON and OFF sessions was counterbalanced.

A CONSORT diagram showing key stages of the study design is presented in Fig. 1.

Fig. 1

CONSORT diagram showing key stages of study design

Clinical trial authorization

Clinical trial authorization under The Medicines for Human Use (Clinical Trials) Regulations 2004 S.I. 2004/1031 was received from the Licensing Authority in November 2012.

Ethical approval and consent

The study was conducted in accordance with the ethical principles that have their origins in the Declaration of Helsinki. The patients were sent approved participant invitation letters that included a reply slip and an information sheet for consideration. If a patient indicated interest by sending a positive reply slip, he/she was contacted by the research nurse and invited to a screening session with the chief investigator, who was also the principal investigator in this study.

Written informed consent was obtained at the screening interview by the chief investigator, with the research nurse present as the patient advocate. Patients were given an opportunity to ask any questions. Consent was obtained before any study procedures took place.


Recruitment commenced in April 2013. The target population was males and females aged 50–80 years with idiopathic, sporadic PD as determined by the UK Parkinson’s Disease Society Brain Bank Clinical Diagnostic Criteria, with a Hoehn–Yahr disease severity stage of 1–4 [30].

The target number of complete datasets was 50. The sample size was determined in terms of obtaining sufficiently precise estimates (as represented by the width of a 95% confidence interval) of key parameters that would inform a power calculation for a subsequent main trial. A figure of 50 is supported by reports on sample sizes for pilots with continuous outcomes [31, 32].

Eligible patients were identified from the sponsor’s (University Hospital of North Midlands NHS Trust) Department of Neurology using the PD database and clinic lists, the GPs in Staffordshire (acting as participant identification sites), the chief investigator’s private practice, local pharmacies, advertisements in local newspapers and recruitment talks given to local PD associations.

Patients entered the study on one of the two IMPs within the approved dosage range. The maximum dosage of each IMP, as specified in the summary of product characteristics, was pramipexole 0.52–3.15 mg once daily and ropinirole 2–12 mg once daily [33, 34].

Patients were maintained on the same or equivalent therapeutic dose for the stabilization periods and for the ON medication research session, using published conversion norms, unless clinical indications suggested modification [35].

Randomization procedure

Once the consent form had been signed, the chief investigator randomized the patient to the treatment arms.

Third-party randomization, in a 1:1 ratio, took place during the screening clinic visit using a locked system that automated the random assignment of treatment groups to randomization numbers. The third-party randomization protocol was a web-based programme, hosted by Keele University, and was on a password-protected site.

The patient’s randomization code was recorded in the medical records and case report form. This information was stored on-line, was read-only and could be accessed by the chief investigator and research nurse at any point during the trial.

Level and method of blinding

During the trial, the patients and the clinical members of the research team (chief investigator, research nurse) were not blind to the medication codes. The patients could not be blinded as they saw the physical nature of the tablets, and the chief investigator and research nurse managing the patients needed to know how to advise the patients at the beginning of each treatment phase of the trial, and when switching between the IMPs, and how to prepare for the medication withdrawal period.

Only the research assistant administering the cued recall memory test during the ON and OFF research sessions was blind to the medication codes. The medication codes were not made available to the research assistant until each patient had completed the trial, with his/her data scored and entered into the database.


The two primary outcomes were efficacy of processes and procedures used to manage symptoms during the washout period (measured in relation to adverse events) and estimates of cued recall performance (to inform the sample size calculation). The secondary outcomes were rates of consent and missing data, acceptability of the support provided for the OFF medication sessions and whether the OFF session itself was manageable, the experience of switching between IMPs (assessed at the semi-structured interview administered at the end of the trial) and barriers to participation for eligible patients and informal carer givers (semi-structured interview).

Enrolment and study visits

Patients completing the feasibility trial were intended to participate for a maximum period of no longer than 16 weeks. During this period, they were involved in eight separate sessions:

  • Three involved the chief investigator and research nurse and took place during research-dedicated clinics in a UHNM research facility:

    • Screening visit in week 1 (visit 1)

    • Mid-study follow-up appointment, taking place between weeks 6 and 8 (visit 4)

    • An end-of-study follow-up appointment, between weeks 14 and 16 (visit 7)

  • Four involved the research assistant and took place in the patient’s home, around 9 or 10 a.m. in the morning:

    • Separate ON and OFF medication sessions for the first IMP, between weeks 6 and 7 (visits 2 and 3)

    • Separate ON and OFF medication sessions for the second IMP, between weeks 13 and 15 (visits 5 and 6)

  • One visit with the research assistant followed the end-of-study clinic visit, for the purposes of completing a semi-structured interview about participation, between weeks 14 and 16 (visit 8).

For the ON research session, medication was taken as usual roughly 60 min before the session commenced. For the OFF session, medication was delayed prior to the session. Medication was resumed following completion of the session, according to criteria discussed with the chief investigator. The ON medication and OFF medication sessions for each drug should have taken place during a 7-day window, on separate days separated by at least 3 days. The order of the ON and OFF research sessions was determined by the randomization programme.

Washout periods

A single washout period, between the first and second IMP, was used to eliminate a carryover effect. The elimination half-life for each drug is 8–12 h for pramipexole in older patients, falling at the upper end of the range: 6 h for ropinirole and 3 h for l-dopa and monoamine oxidase B inhibitors.

To achieve 93.75% elimination of each drug (equating to four half-lives), the following washout periods were required: pramipexole, 48 h (4 × 12 h); ropinirole, 24 h (4 × 6 h); l-dopa and monoamine oxidase B inhibitors, 12 h (4 × 3 h). At the mid-study clinic visit, patients were given the second IMP, which they took until the end-of-study visit.

During the washout period, patients continued to take l-dopa and/or monoamine oxidase B inhibitor as usual. The half-lives of these drugs are shorter than those of the IMPs, and therefore, the washout period required for 93.75% elimination of l-dopa and monoamine oxidase inhibitor (half-life is between 2 and 3 h) is 12 h. l-dopa and monoamine oxidase inhibitors were withdrawn 12 h (overnight) before the OFF research session. The OFF session took place in the morning, following a washout period resulting in four half-lives of the drug being eliminated (i.e. 93.75%).

Data security and monitoring

A research risk assessment of the feasibility trial was carried out by the chief investigator and the regulatory research associate from the sponsor’s research and development department, prior to the preparation of the protocol. The first risk assessment was assessed as moderate by 1 point initially (1 point over the low to moderate risk boundary), but reassessed later as low-risk, requiring minimal mitigation.

Validation procedures included regular data entry checks performed on a minimum of 25% of complete patient data by the monitor. All source data and trial documentation were available for trial-related monitoring. The patients consented for this within the consent process.

Patients’ contact details were disclosed to the research assistant, for the purposes of arranging the four home visits to complete the memory assessment. All patient data collected by the research assistant were identified using a unique study code. This same code was used to enter patient data into the Microsoft Excel© database. Copies of signed informed consent forms were placed in the patient’s medical records and originals retained in the trial file and kept separate from the data.

Monitoring procedures were carried out by the sponsor and were in accordance with standard operating procedures. The monitor checked the case report forms, trial master file and other trial documentation for completeness and the compliance of the trial team with the protocol and good clinical practice (GCP) standards. The initial monitoring visit occurred before the trial started to ensure that the trial complied with the protocol and GCP standards. Further monitoring visits took place at regular intervals (approximately twice per year), with a final monitoring visit conducted to close the trial.

Study committees

A steering group was formed comprising a lay chair, lead researcher (Edelstyn), chief investigator (Ellis), research nurse representatives, research assistant (Shepherd), pharmacy representatives, research and development department representatives (Longshaw, Watts) and up to four patient-public representatives.

Membership of the operational group included the lead researcher (chair), chief investigator, research nurse representatives, research assistant, trial pharmacists, sponsor research and development representatives. The operational group met on a monthly basis.

Analytic strategy

Quantitative data

As this was a feasibility study, no hypothesis tests were performed. Instead, 95% confidence intervals (CIs) are presented to indicate the precision of calculated estimates and to present a range of plausible alternative values for these estimates.

Qualitative data

All patients who completed the trial were invited to participate in semi-structured interview to reflect on their experience of participation.

A barriers-to-participation study was introduced 9 months into the 18-month recruitment period to explore reasons for poor recruitment. NRES permission for retrospective contact of decliners prior to receiving approval was not obtained. There was no a priori selection of decliners for interview. All those who agreed were interviewed.

Interview recordings were transcribed by the research assistant. During the transcription process, initial thoughts and observation were noted. Accuracy was checked by listening to each recording while reading through the respective transcript, and any necessary amendments were made. The interviews were transcribed into Microsoft Word© and were analysed using thematic analysis [36].

Initial topic coding was achieved by reading through the transcripts and detailing basic themes within the text using the Microsoft Word© comment function. New themes emerged from the data throughout this process, and the procedure was therefore repeated to ensure that text from transcripts that had been read prior to newly developed themes could be subsumed under the appropriate theme.


Recruitment and retention

A total of 220 patients with PD were screened for eligibility (number of patients with PD found among neurology patients screened). One hundred and forty-five were excluded, and 75 eligible patients were invited to take part. Fifty-three patients declined, 22 consented and 16 completed the study. The age distribution at the time of consent is shown in Table 1.

Table 1 Age distribution (at time of consent)

Information on the number of patients assessed for eligibility, randomized and discontinued (together with reasons) and the complete datasets analysed are presented in a CONSORT diagram in Fig. 2. Ten patients were randomized to treatment arm 1 (pramipexole then ropinirole) and 12 to treatment arm 2 (ropinirole then pramipexole).

Fig. 2

CONSORT diagram showing the number of patients assessed for eligibility, randomized, follow-up and complete datasets analysed


No serious adverse events were reported. Effects of short-term withdrawal of the IMPs were minimal when assessed at the mid- and end-of-study visits. A total of 84 non-serious adverse events were reported (plus one report of medication error), with seven participants experiencing adverse events; as a result, two withdrew from the study and three were swapped back to their original dopamine agonist but agreed to stay in the study. There was one additional case of the IMP being temporarily stopped due to an adverse event. This enabled the testing and refinement of adverse event handling and reporting by the clinical members of the research team. In terms of adverse events (non-serious events considered to be related to the IMP by the chief investigator), the rate was very similar between both groups (pramipexole, n = 28; ropinirole, n = 30).

Allocation to treatment arm, IMP daily dose, duration of IMP treatment and details of compliance for each patient are provided in Table 2. Arm 1 had 3 patients who discontinued their assigned treatment due to non-serious adverse events (PD1, PD11, PD21). These patients were switched to their original dopamine agonist but agreed to remain in the study to continue the study processes. Other patients who deviated from their assigned IMP were PD2 (pramipexole was temporarily stopped for 8 days, and the patient switched back to own pre-study supply of ropinirole prior to the mid-study visit with the chief investigator) and PD9, who ran out of study medication prior to the mid- and end-of-study visits and took own supply (however, this patient was receiving the correct IMPs for the testing sessions). Therefore, PD2, PD6, PD9, PD10, PD14, PD16 and PD19 fully completed the study.

Table 2 Pre-study dopamine agonist, allocated treatment arm, first and second IMP and daily dose and duration of IMP treatments

Arm 2 had 3 patients who discontinued their assigned treatment due to non-serious adverse events (PD7, PD18 and PD23). Participant PD7 reverted to his original dopamine agonist due to adverse events but agreed to remain in the study to continue the study processes. However, PD18 and PD23 wished to fully withdraw and therefore had no further study assessments or visits. Other participants who deviated from their assigned IMP were PD24, who discontinued pramipexole due to non-serious adverse events but after the ON/OFF research session (therefore, this participant was on ropinirole, not pramipexole, for the end-of-study visit), and PD17, who switched medications from pramipexole to ropinirole prior to the end-of-study visit (but after the ON/OFF session due to attending a private (non-research) appointment). Therefore PD3, PD4, PD8, PD12, PD17, PD20, PD22, PD24 and PD25 fully completed the study.

In total, therefore, 16 participants completed the study (defined as completing the memory assessment sessions on their assigned IMP) and 5 completed the end-of-study interviews with the research assistant.

Owing to the small sample size, the estimates from the study are necessarily imprecise (as reflected in wide confidence intervals). However, the data suggest that the effect of pramipexole on cued recall (OFF–ON difference score) is 0.030 (95% CI −0.192, 0.079). The corresponding effect of ropinirole is 0.007 (95% CI −0.070, 0.084), indicating a somewhat smaller decrement in recall. It also appears, from Table 3 and Fig. 3, that patients entering the study on pramipexole are susceptible to both a pramipexole-related (OFF–ON difference score 0.034, 95% CI −0.067, 0.135) and ropinirole-related (OFF–ON difference score 0.036, 95% CI −0.144, 0.215) decrement in cued recall. For those entering the study on ropinirole, pramipexole (OFF–ON difference score 0.027, 95% CI −0.038, 0.091) showed a decrement in cued recall, but this did not occur with ropinirole (OFF–ON difference score −0.016, 95% CI −0.092, 0.061).

Table 3 Effect of pramipexole versus ropinirole on cued recall according to patients’ pre-study dopamine agonist
Fig. 3

Effect of pramipexole (PPX) versus ropinirole (RPR) on cued recall according to patients’ dopamine agonist on entry to the study

If the effects of pramipexole and ropinirole are compared (pramipexole minus ropinirole), there is a small difference favouring ropinirole (0.023, 95% CI −0.088, 0.134). A crossover trial is potentially subject to a period effect, and this was assessed by comparing the difference in effect of the two IMPs, between the first and second period. The difference was 0.022 (95% CI −0.094, 0.138). The estimate of pramipexole versus that of ropinirole adjusted for the period effect is slightly smaller than the crude estimate, 0.020, 95% CI −0.095, 0.136.

Accordingly, in addition to an analysis adjusting for a period effect, it would be advisable to utilize blocked randomization in a future trial, whereby equal numbers of patients would be allocated to each treatment sequence, helping to offset any period effect. This also raises the issue of including an additional washout period, prior to administration of the first treatment, in order to remove the potential effect of pre-randomization IMP.

The standard deviation (SD) of the within-subject differences between the two IMPs was 0.208 (95% CI 0.154, 0.322). If this is inflated by approximately 25% to give 0.260 as a conservative estimate of the SD in a subsequent definitive trial [30], Table 4 gives the numbers of subjects required to detect a range of differences in a fully powered RCT, with statistical power of 0.90 and a two-tailed significance level of p ≤ 0.05.

Table 4 Sample sizes for a main study according to a range of effects

The minimum and maximum OFF medication cued recall scores for either ropinirole or pramipexole were 0.040 and 0.735, giving a range of 0.695. Assuming an effect of at least 10% of the observed range of scores (0.070) to be clinically important, a main study would therefore require a sample size of just under 150, at the above levels of power and statistical significance (without accounting for loss to follow-up).

Secondary outcomes

With regard to the secondary objectives, the consent and missing data rates were 29% (95% CI 19%, 40%) and 27% (95% CI 9%, 46%), respectively.

The experience of participation from the end of study interviews with 5 patients completing the study revealed two main themes, centred on the experience of participation and the motivation to take part. The washout period itself, as well as the preparation for the OFF sessions, were acceptable, and the experience of switching between the IMPs was manageable. Two main themes were identified: the experience of participating and motivation to take part. Both themes, together with each of the subthemes, are presented in Table 5. All patients contributed to each of the two main themes, but not necessarily to all subthemes.

Table 5 The main themes and subthemes and illustrative quotations from the end-of-study interviews with 5 patients

Finally, barriers to participation were explored with 5 patients who declined to take part in the main study and 5 informal carer givers. Four main themes emerged, which focused on medication concerns relating to stability and side effects, accessibility of the recruitment materials, fear of the unknown and carer workload. The four main themes together with each of the subthemes, along with illustrating quotations, are presented in Table 6. All participants contributed to each of the four main themes, but not necessarily to all subthemes.

Table 6 The main themes and subthemes and illustrative quotations from the barriers-to-participation interviews with 5 patients who declined to take part and 5 informal caregivers


This study presented challenges to recruitment in both design and execution, and while it was a major aim of the study to assess this, evaluation of these challenges also provided the opportunity to explore how these could be overcome for future studies in this area. The study necessitated the use of different methods and approaches to identify and recruit eligible patient-participants. This included raising awareness using promotional material in local newspapers and pharmacies and establishing links with GP surgeries (Participant Identification Centres) through the Primary Care Research Network. However, the recruitment target of 50 was not met. Consequently, estimates of key parameters are somewhat imprecise; in particular, the wide confidence interval around the estimated treatment effect between the two IMPs suggests that this effect could plausibly lie between approximately –0.10 and 0.14; the suggested minimum clinically important effect of 0.70 lies within this range but is somewhat larger than the point estimate of approximately 0.20. The small sample size also means that the probability of detecting adverse events is reduced [37].

The challenges and mitigations implemented included the development of a suite of study-specific standard operating procedures, including a named standard operating procedure controller. Infrastructure to support clinical trials of medicinal products was not optimum at study start-up. The organization underwent significant changes prior to and during the study. We now have clinical studies officers in place to support study set-up, which includes early engagement with support departments and academics that lead clinical trials of medicinal products (this includes a full risk assessment by the sponsor, which is now used to support the decision regarding capability/capacity to sponsor clinical trials of medicinal products). There are also dedicated study monitor roles within the department. The barriers-to-participation study identified four main themes: concerns that changing medication may worsen their PD symptoms and also fears about side effects; trial accessibility, e.g. the information sheet was long and too technical; fear of the research process; and worries about increasing the work load of spouses/informal carers.

Recruitment challenges are particularly relevant in older populations [38], and the heterogeneous nature of PD means that achieving a sufficient homogeneous patient sample requires the implementation of very effective recruitment strategies. Individuals with PD are typically elderly and disabled; therefore, participation in clinical trials often requires the assistance of a caregiver, not only with hospital visits and organizing medication but also in the decision to participate itself.

For drug trials in particular, patients with PD have positive perceptions of placebo-controlled trials, although they are strongly in favour of receiving the active agent [39]. Furthermore, evidence suggests a preference for trial designs that include two active agents (or an active comparator) over placebo-controlled designs and indicates that this may facilitate recruitment due to patients being less likely to experience ‘lessebo’ effects (the negative expectations associated to the administration of a placebo) [40].

The challenges of conducting research in PD are illustrated by a recent on-line 5-item survey by Mathur et al. [41]. Their aim was to explore patients’ barriers to participation and researchers’ challenges in conducting drug trials in PD. From an estimated sample size exceeding 10,000 people, the proportion of respondents was around 3%. A total of 33 responses were from researchers and 263 from patients. From the researcher perspective, the main concerns were related to insufficient financial and administrative support. Of greater interest to our study was the overlap with Mathur et al.’s patient-reported barriers: fear of potential adverse consequences, misconceptions regarding clinical trial systems, and issues related to a perceived lack of communication of relevant information between the research and patient communities.

To address the generic as well as specific recruitment problems discussed above, we recommend adoption of the the ‘QuinteT Recruitment Intervention’ (QRI), which aims to understand recruitment as it happens and then develop an action plan to address identified difficulties and optimize informed consent in collaboration with the CTU and chief investigator [41]. The QRI uses a combination of standard and innovative qualitative research methods with some simple quantification to understand recruitment and identify sources of difficulties.


Recruitment rates to the main feasibility study were disappointing. Patient and informal caregiver concerns clearly influenced this and can, at least to some extent, be mitigated by improved communication, reassurance, exposure to the research team and awareness-raising. The sponsor now has improved capability to support CTIMPs. Going forward, the sponsor together with the research team is in a position to mitigate these challenges in a subsequent main trial, particularly in collaboration with a Clinical Trials Unit. Implementing these measures, together with integration of the QRI from the outset in order to prevent difficulties developing, and optimizing recruitment from the start, rather than applying it to an ongoing RCT experiencing recruitment shortfalls where time to rectify/address recruitment difficulties may be limited, and increase confidence that realistic recruitment targets, while still challenging, could be achieved.

Delivering safe, effective and cost-effective pharmacological management of PD is a priority. It is important to communicate effectively to the patient community the importance of drug trials in developing new treatments and to engage patients and informal caregivers in the communication and promotion of research.

It is important to note that this feasibility study does not provide and did not seek to provide firm evidence that dopaminergic medication affects memory. Therefore, people with PD should not change their medication based on the results of this study.

Change history

  • 24 October 2017

    A correction to this article has been published.



Confidence interval


Good clinical practice


Investigational medicinal product


Parkinson’s disease


Standard deviation


  1. 1.

    Parkinson's UK. Parkinson’s prevalence in in the United Kingdom. London: Parkinson’s UK. 2009.

  2. 2.

    Office for National Statistics. [].

  3. 3.

    Wickremaratchi M, Perera D, O’Loghlen C, Sastry D, Morgan E, Jones A, Edwards P, Robertson N, Butler C, Morris H, Ben-Shlomo Y. Prevalence and age of onset of Parkinson’s disease in Cardiff: a community based cross sectional study and meta-analysis. J Neurol Neurosurg Psychiatry. 2009;80:805–7.

    CAS  Article  PubMed  Google Scholar 

  4. 4.

    House of Commons Library Research. The ageing population. Key issues for the new parliament. 2010.

    Google Scholar 

  5. 5.

    National Institute for Health and Care Excellence. Parkinson’s disease in over 20s: diagnosis and management. [].

  6. 6.

    Samii A, Nutt J, Ransom B. Parkinson’s disease. Lancet. 2004;363:1783–93.

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    Hornykiewicz O. Dopamine (3-hydroxytyramine) and brain function. Pharmacol Rev. 1966;9:925–64.

    Google Scholar 

  8. 8.

    Obeso J, Rodríguez-Oroz M, Benitez-Temino B, Blesa F, Guridi J, Marin C, Rodriguez M. Functional organization of the basal ganglia: therapeutic implications for Parkinson’s disease. Mov Disord. 2008;23:S548–59.

    Article  PubMed  Google Scholar 

  9. 9.

    Fearnley J, Lees A. Ageing and Parkinson’s disease: substantia nigra regional selectivity. Brain. 1991;114:2283–301.

    Article  PubMed  Google Scholar 

  10. 10.

    Jankovic J. Pathophysiology and clinical assessment of Parkinsonian symptoms and signs. In: Pahwa R, Lyons KE, Koller WC, editors. Handbook of Parkinson’s Disease. 3rd ed. New York: Marcel Dekker; 2003. p. 71–108.

    Google Scholar 

  11. 11.

    Montgomery Jr EB. Two advances in the management of Parkinson disease. Cleve Clin J Med. 2002;69(8):639–43.

    Article  PubMed  Google Scholar 

  12. 12.

    Mungersdorf M, Sommer U, Sommer M, Reichmann H. High-dose therapy with ropinirole in patients with Parkinson’s disease. J Neural Transm. 2001;108:1309–17.

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    Chung J, Wu A, Lew M. Medications. In: Pahwa R, Lyons K, Koller W, editors. Handbook of Parkinson’s disease. 3rd ed. New York: Marcel Dekker; 2003. p. 363–452.

    Google Scholar 

  14. 14.

    Dopamine agonists in Parkinson’s disease: special focus on pramipexole PR [].

  15. 15.

    Junghanns S, Glockler T, Reichmann H. Switching and combining of dopamine agonists. J Neurol. 2004;251 suppl 6:vi19–vi23.

    Google Scholar 

  16. 16.

    Cools R. Dopaminergic modulation of cognitive function-implications for l-DOPA treatment in Parkinson’s disease. Neurosci Biobehav Rev. 2006;30:1–23.

    CAS  Article  PubMed  Google Scholar 

  17. 17.

    Cools R, Altamirano L, D’Esposito M. Reversal learning in Parkinson’s disease depends on medication status and outcome valence. Neuropsychologia. 2006;44:1663–73.

    Article  PubMed  Google Scholar 

  18. 18.

    Jahanshahi M, Wilkinson L, Gahir H, Dharmarinda A, Lagnado D. Corrigendum to “Medication impairs probabilistic classification learning in Parkinson’s disease” [Neuropsychologia 48 (2010) 1096–1103]. Neuropsychologia. 2011;49:3517.

    Article  Google Scholar 

  19. 19.

    Rowe J, Hughes L, Ghosh B, Eckstein D, Williams-Gray C, Fallon S, Barker R, Owen A. Parkinson’s disease and dopaminergic therapy—differential effects on movement, reward and cognition. Brain. 2008;131:2094–105.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  20. 20.

    Shook S, Franz E, Higginson C, Wheelock V, Sigvardt K. Dopamine dependency of cognitive switching and response repetition effects in Parkinson’s patients. Neuropsychologia. 2005;43:1990–9.

    Article  PubMed  Google Scholar 

  21. 21.

    Martinez-Martin P, Schapira A, Stocchi F, Sethi K, Odin P, MacPhee G, Brown R, Naidu Y, Clayton L, Abe K, Tsuboi Y, MacMahon D, Barone P, Rabey M, Bonuccelli U, Forbes A, Breen K, Tluk S, Olanow C, Thomas S, Rye D, Hand A, Williams A, Ondo W, Chaudhuri K. Prevalence of nonmotor symptoms in Parkinson’s disease in an international setting: study using nonmotor symptoms questionnaire in 545 patients. Mov Disord. 2007;22:1623–9.

    Article  PubMed  Google Scholar 

  22. 22.

    Pont-Sunyer C, Hotter A, Gaig C, Seppi K, Compta Y, Katzenschlager R, Mas N, Hofeneder D, Brücke T, Bayés A, Wenzel K, Infante J, Zach H, Pirker W, Posada I, Álvarez R, Ispierto L, De Fàbregues O, Callén A, Palasí A, Aguilar M, Martí M, Valldeoriola F, Salamero M, Poewe W, Tolosa E. The Onset of Nonmotor Symptoms in Parkinson’s disease (The ONSET PD study). Mov Disord. 2014;30:229–37.

    Article  PubMed  Google Scholar 

  23. 23.

    Cohn M, Giannoylis I, De Belder M, Saint-Cyr J, McAndrews M. Associative reinstatement memory measures hippocampal function in Parkinson’s disease. Neuropsychologia. 2016;90:25–32.

    Article  PubMed  Google Scholar 

  24. 24.

    Edelstyn N, Mayes A, Condon L, Tunnicliffe M, Ellis S. Recognition, recollection, familiarity and executive function in medicated patients with moderate Parkinson’s disease. J Neuropsychol. 2007;1:131–47.

    Article  PubMed  Google Scholar 

  25. 25.

    Edelstyn N, John C, Shepherd T, Drakeford J, Clark-Carter D, Ellis S, Mayes A. Evidence of an amnesia-like cued-recall memory impairment in nondementing idiopathic Parkinson’s disease. Cortex. 2015;71:85–101.

    Article  PubMed  Google Scholar 

  26. 26.

    Edelstyn N, Shepherd T, Mayes A, Sherman S, Ellis S. Effect of disease severity and dopaminergic medication on recollection and familiarity in patients with idiopathic nondementing Parkinson’s. Neuropsychologia. 2010;48:1367–75.

    Article  PubMed  Google Scholar 

  27. 27.

    Shepherd T, Edelstyn N, Mayes A, Ellis S. Second-generation dopamine agonists and recollection impairments in Parkinson’s disease. J Neuropsychol. 2013;7:284–305.

    Article  PubMed  Google Scholar 

  28. 28.

    Eldridge SM, Chan CL, Campbell MJ, Bond CM, Hopewell S, Thabane L, Lancaster GA, on behalf of the PAFS consensus group. CONSORT 2010 statement: extension to randomised pilot and feasibility trials. Pilot Feasibility Stud. 2016;2:64–732.

    Article  PubMed  PubMed Central  Google Scholar 

  29. 29.

    Hoehn M, Yahr M. Parkinsonism: onset, progression, and mortality. Neurology. 1967;17:427–42.

    CAS  Article  PubMed  Google Scholar 

  30. 30.

    Sim J, Lewis M. The size of a pilot study for a clinical trial should be calculated in relation to considerations of precision and efficiency. J Clin Epidemiol. 2012;65:301–8.

    Article  PubMed  Google Scholar 

  31. 31.

    Whitehead A, Julious S, Cooper C, Campbell M. Estimating the sample size for a pilot randomised trial to minimise the overall trial sample size for the external pilot and main trial for a continuous outcome variable. Stat Methods Med Res. 2015;25:1057–73.

    Article  PubMed  PubMed Central  Google Scholar 

  32. 32.

    Requip XL prolonged-release tables. Summary of product characteristics. [].

  33. 33.

    Mirapexin prolonged-release tables. Summary of product characteristics. [].

  34. 34.

    Lyons K, Pahwa R. An open-label conversion study of pramipexole to ropinirole prolonged release in Parkinson’s disease. Mov Disord. 2009;24:2121–7.

    Article  PubMed  Google Scholar 

  35. 35.

    Braun V, Clarke V. Using thematic analysis in psychology. Qual Res Psychol. 2006;3:77–101.

    Article  Google Scholar 

  36. 36.

    Viechtbauer W, Smits L, Kotz D, Budé L, Spigt M, Serroyen J, Crutzen R. A simple formula for the calculation of sample size in pilot studies. J Clin Epidemiol. 2015;6:1375–9.

    Article  Google Scholar 

  37. 37.

    Bartlam B, Crome P, Lally F, Beswick AD, Cherubini A, Clarfiled M, Edbrooke D, Farre A, Hertogh C, Lesauskaite V, Mills G, Muller M, Oristell J, Prada GI, Ruggero C, Sinclair-Cohen J, Topinkova E, Zaleqski Z. The views of older people and carers on participation in clinical trials: the PREDICT study. Clin Investig. 2012;2:327–36.

    Article  Google Scholar 

  38. 38.

    Valades A, Coelho M, Mestre T, Guedes FC, Finisterra M, Noronha A. What motivates Parkinson’s disease patients to enter clinical trials? Parkinsonism Relat Disord. 2011;17:667–71.

    Article  Google Scholar 

  39. 39.

    Mestre TA, Shah P, Marras C, Tomlinson G, Lang AE. Another face of placebo: the lessebo effect in Parkinson’s disease: meta-analyses. Neurology. 2014;82:1402–9.

    Article  PubMed  PubMed Central  Google Scholar 

  40. 40.

    Mathur S, DeWitte S, Robledo I, Isaacs T, Stamford J. Rising to the challenges of clinical trial improvement in Parkinson’s disease. J Parkinsons Dis. 2015;5:263–8.

    Article  PubMed  Google Scholar 

  41. 41.

    Donovan JL, Rooshenas L, Jepson M, Elliott D, Wade J, Avery K, Mills N, Wilson C, Paramasivan S, Blazeby JM. Optimising recruitment and informed consent in randomised controlled trials: the development and implementation of the Quintet Recruitment Intervention (QRI). Trials. 2016;17:283–94.

    Article  PubMed  PubMed Central  Google Scholar 

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This study presents independent research funded by the National Institute for Health Research—Research for Patient Benefit (RfPB) Programme (PB-PG-0211-24101). The views expressed are those of the authors and not necessarily those of the NHS, the National Institute for Health Research or the Department of Health.

Availability of data and materials

The datasets generated during the current study were posted on the EUdract website repository on 4 March 2016 and can be found here:

Authors’ contributions

NMJE drafted the manuscript. SJE and LL collated the clinical data related to the pre-study dopamine agonist, allocation to treatment arm, first and second IMP and daily dose and duration of IMP treatments. TAS collected the memory data and conducted the semi-structured interviews. TAS and MM analysed and interpreted the qualitative data. JS analysed and interpreted the primary outcome memory data and rates of consent and missing data. All authors read and commented on the earlier drafts and approved the final version.

Competing interests

The authors declare that they have no competing interests.

Consent for publication

Consent to publish anonymized data and direct quotations from semi-structured interviews in scientific and medical journals was also obtained.

Ethics approval and consent to participate

Ethics approval was obtained from The Health Research Authority National Research Ethics Service Committee North West—Greater Manchester Central (Reference 13/NW/0009)—in February 2013. Each participant provided full consent to take part in the study. All participants provided full informed consent.

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Corresponding author

Correspondence to Nicola M. J. Edelstyn.

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A correction to this article is available online at

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Shepherd, T.A., Edelstyn, N.M.J., Longshaw, L. et al. Feasibility of a randomized single-blind crossover trial to assess the effects of the second-generation slow-release dopamine agonists pramipexole and ropinirole on cued recall memory in idiopathic mild or moderate Parkinson’s disease without cognitive impairment. Pilot Feasibility Stud 4, 11 (2018).

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  • Feasibility study
  • Crossover
  • Medication withdrawal
  • Pramipexole PR
  • Ropinirole MR
  • Idiopathic Parkinson’s disease
  • Cued recall
  • Safety processes
  • Acceptability
  • Barriers to participation