Table 1 Study schedule of enrollment, interventions, and assessments

Enrollment

 Eligibility screening

X

 Written informed consent

X

 Randomization and allocation (after eligibility criteria met and written informed consent obtained)

X

Interventions

 First stage: L. reuteri vs placebo

X

 Second stage: L. reteri plus OXT spray vs placebo plus OXT spray

X

Assessments

 Past medical history and medications

X

 Surveys (ABC, SRS-2, GSI, and microbiome)

X

X

X

 Labs (OXT and fecal microbiome)

X

X

X

 Tests (emotion matching and eye-tracking)

X

X

X

 Adverse events

X

X

X